Abstract

Author:
Md. Mokarram Hossain, Md. Rashidul Islam, Ronzon Chandra Das, Md. Mahbubul Haque, Mohammad Delwar Hossain and Faria-Ter Jerin

Doi: 10.26480/trab.02.2024.106.109

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Brown rot of potato is caused by a soil borne bacterium, Ralstonia solanacearum(Smith), a major limiting factor in the production of many crop plants around the world. An experiment was conducted to assess the status of brown rot disease in potatoes with the development of a rapid detection technique and approaches for production of brown rot free export quality potatoes to boost up potato export from Bangladesh. Survey results revealed a variation in latent infection (%) in tuber samples collected from six major growing areas viz. Dinajpur, Rangpur, Bogra, Joypurhat, Munshigonj and Comilla. The lowest (30%) latent infection was detected in tuber samples collected from Joypurhat as compared to Rangpur and Munshigonj in case of Asterix. However, in Diamant, the lowest (40%) latent infection was observed in Rangpur while 80% latent infection was recorded in Dinajpur and 88% was recorded in Munshigonj. Intriguingly, no latent infection was detected in Cardinal collected from Dinajpur while 40% latent infection was detected in samples from Joypurhat. However, 60% latent infection was detected in Granola which was collected from Dinajpur. The detection of R. solanacearum was reported in latently infected tubers by PCR using primers corresponding to 16SrDNA. In this study, PCR with primers PS-1 and PS-2 (16S rDNA) was used to detect R. solanacearum in latently infected tubers. An amplicon size corresponding to 553bp were obtained using enriched tuber extract as template.